What are Vacutainer Tubes?
These are the colour-coded plastic tubes with a rubber stopper. They are used to collect blood for various investigation
What is the color significance?
Different colors represent different uses:
Red: No anticoagulant, used for serological examination in biochemistry
Grey: Contains Sodium Fluoride, Used for glucose estimation
Lavender/ purple: contains EDTA, used in hematological examinations like complete hemogram, ESR,
Light Blue: contains 3.2% sodium citrate, used for coagulation studies like PT, APTT
Green: contains heparin, used for bone marrow studies
Yellow: Contains citrate, used for blood culture
Black: Commonly used for ESR
What is the history of Vacutainer Tubes?
Vacutainer tubes were invented by Joseph Kleiner in 1949. Vacutainer is a registered trademark of Becton Dickinson, which manufactures and sells the tubes today.
How is the Vacutainer tube used?
When a tube is inserted into the holder, its rubber cap is punctured by this inner needle and the vacuum in the tube pulls blood through the needle and into the tube. The filled tube is then removed and another can be inserted and filled the same way. The amount of air evacuated from the tube predetermines how much blood will fill the tube before blood stops flowing.
The term order of draw refers to the sequence in which tubes should be filled. The needle which pierces the tubes can carry additives from one tube into the next, so the sequence is standardized so that any cross-contamination of additives will not affect laboratory results.
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